Two of a kind: China’s first pet cloning service duplicates star canine

Owner He Jun poses with his dogs, nine-year-old Juice and its two-month-old clone, at his pet resort in Beijing, China November 26, 2018. Picture taken November 26, 2018. REUTERS/Jason Lee

By Joseph Campbell

BEIJING (Reuters) – Juice is a one-foot tall canine wonder who has starred in dozens of Chinese film and television productions.

As he gets older and his illustrious career peaks, his Beijing-based master has one wish for the mutt – to live on. Maybe forever.

A mongrel stray adopted off the streets, the nine-year-old Juice — or “Guozhi” in Mandarin — is unable to reproduce since he was neutered from an early age. But his master, animal trainer He Jun, wants to continue his star pooch’s image by making a genetic clone.

“Juice himself is a piece of intellectual property with social influence,” said He.

To achieve that, He went to Sinogene, China’s first biotech company to provide pet cloning services. Sinogene made headlines when it successfully cloned a gene-edited beagle in May last year. A month later, it launched commercial cloning services.

For at least 380,000 yuan ($55,065), pet owners can clone their pets.

Sinogene’s CEO Mi Jidong said the company’s pet cloning business is in its initial stages, but he plans to expand services to eventually include gene editing.

“We’ve discovered that more and more pet owners want their pets to accompany them for an even longer period of time,” said Mi.

China’s biotech industry is growing rapidly and, compared with similar enterprises in the West, faces relatively few regulatory barriers.

Earlier this year, a Shanghai lab produced the world’s first monkey clones, two long-tailed macaques. More controversially, He Jiankui of China’s Southern University of Science and Technology last month claimed he used gene-editing technology to alter the embryonic genes of twin girls.

Tin-Lap Lee, an associate professor of biomedical sciences at the Chinese University of Hong Kong, said while China has regulations on the use of animals for lab research, there are no laws explicitly covering animal cloning.

“On the government side, the image of this cloning industry is very high-tech, and definitely…is very supportive of those high-tech industries because of their high-profit-margin,” said Lee.

In Juice’s case, skin samples were collected from the dog’s lower abdomen and within weeks, Sinogene was able to isolate his DNA and fertilize an egg.

The fertilized egg is then surgically inserted into the uterus of a surrogate mother dog – in this case, a beagle.

Juice’s copy, “Little Juice” — or “Zhizhi” in Chinese — was born in mid-September and stayed with its surrogate mother in Sinogene’s lab for about a month. The puppy was later given to He at a small ceremony at which the original Juice was present.

While He has not committed Little Juice to show business just yet, he sees lots of potential.

“We believe he’ll be even better than the older Juice,” He said.

(Reporting by Joseph Campbell; Editing by Sam Holmes)

Chinese scientists break key barrier by cloning monkeys

Zhong Zhong and Hua Hua, two cloned long tailed macaque monkeys are seen at the Non-Primate facility at the Chinese Academy of Sciences in Shanghai, China January 10, 2018.

By Ben Hirschler

LONDON (Reuters) – Chinese scientists have cloned monkeys using the same technique that produced Dolly the sheep two decades ago, breaking a technical barrier that could open the door to copying humans.

Zhong Zhong and Hua Hua, two identical long-tailed macaques, were born eight and six weeks ago, making them the first primates — the order of mammals that includes monkeys, apes and humans — to be cloned from a non-embryonic cell.

It was achieved through a process called somatic cell nuclear transfer (SCNT), which involves transferring the nucleus of a cell, which includes its DNA, into an egg which has had its nucleus removed.

Researchers at the Chinese Academy of Sciences Institute of Neuroscience in Shanghai said their work should be a boon to medical research by making it possible to study diseases in populations of genetically uniform monkeys.

But it also brings the feasibility of cloning to the doorstep of our own species.

“Humans are primates. So (for) the cloning of primate species, including humans, the technical barrier is now broken,” Muming Poo, who helped supervise the program at the institute, told reporters in a conference call.

“The reason … we broke this barrier is to produce animal models that are useful for medicine, for human health. There is no intention to apply this method to humans.”

Genetically identical animals are useful in research because confounding factors caused by genetic variability in non-cloned animals can complicate experiments. They could be used to test new drugs for a range of diseases before clinical use.

The two newborns are now being bottle fed and are growing normally. The researchers said they expect more macaque clones to be born over the coming months.

Since Dolly – cloning’s poster child – was born in Scotland in 1996, scientists have successfully used SCNT to clone more than 20 other species, including cows, pigs, dogs, rabbits, rats and mice.

Similar work in primates, however, had always failed, leading some experts to wonder if primates were resistant.

The new research, published on Wednesday in the journal Cell, shows that is not the case. The Chinese team succeeded, after many attempts, by using modulators to switch on or off certain genes that were inhibiting embryo development.

Even so, their success rate was extremely low and the technique worked only when nuclei were transferred from foetal cells, rather than adult ones, as was the case with Dolly. In all, it took 127 eggs to produce two live macaque births.

“It remains a very inefficient and hazardous procedure,” said Robin Lovell-Badge, a cloning expert at the Francis Crick Institute in London, who was not involved in the Chinese work.

“The work in this paper is not a stepping-stone to establishing methods for obtaining live born human clones. This clearly remains a very foolish thing to attempt.”

The research underscores China’s increasingly important role at the cutting-edge of biosciences, where its scientists have at times pushed ethical boundaries.

Three years ago, for example, researchers at Sun Yat-sen University in Guangzhou caused a furor when they reported carrying out the first experiment to edit the DNA of human embryos, although similar work has now been done in the United States.

Scientists at the Shanghai institute said they followed international guidelines for animal research set by the U.S. National Institutes of Health, but called for a debate on what should or should not be acceptable practice in primate cloning.

(Reporting by Ben Hirschler; Editing by Peter Graff)